evaluation of oprd gene expression in carbapenem-resistant pseudomonas aeruginosa strains isolated from severe burn patients with secondary infection
نویسندگان
چکیده
conclusions the results of this study indicated that a decrease in oprd gene expression is an important mechanism of resistance in carbapenem resistant pseudomonas aeruginosa isolated from severe burn patients with secondary infection. results our study showed that 94.2% of the isolates were resistant to imipenem, 99.5% to meropenem, and all were resistant to ertapenem. the oprd gene expression among carbapenem resistant pseudomonas aeruginosa isolates showed a 2 × 10-3 to 0.5 times decrease compared to the standard sensitive strain (p < 0.05). patients and methods one-hundred and eighty-nine clinical isolates of carbapenem resistant pseudomonas aeruginosa, isolated from burn patients, were identified by microbiological methods followed by determination of antibiotic resistance pattern by the kirby-bauer procedure. the expression of oprd gene was determined by quantitative real-time polymerase chain reaction (pcr). objectives this study was performed to investigate the prevalence of carbapenem-resistant strains of pseudomonas aeruginosa in burn patients with secondary infection and also to evaluate oprd gene expression as a possible resistance mechanism to carbapenem in isolated carbapenem resistant p. aeruginosa strains. background pseudomonas aeruginosa is the most common pathogen isolated from severe burn patients with secondary infection. since high resistance to most types of antibiotics is common among these bacteria, the treatment of infections caused by these agents is very difficult. loss of oprd proteins from the outer membrane of bacterial cells causes a significant decrease in the sensitivity of pseudomonas aeruginosa to carbapenems.
منابع مشابه
Insertional inactivation of oprD in carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Tehran, Iran
In this study, we report the insertion sequence ISPpu21 in the oprD porin gene of carbapenem-resistant Pseudomonas aeruginosa isolates from burn patients in Tehran, Iran. Antibiotic susceptibility tests for P. aeruginosa isolates were determined. Production of metallo-β-lactamases (MBLs) and carbapenemase was evaluated and the β-lactamase-encoding and aminoglycoside-modifying enzyme genes were ...
متن کاملevaluation of ampc gene expression in carbapenem resistant pseudomonas aeruginosa strains isolated from severely burned patients with secondary infection in hospitals
background pseudomonas aeruginosa is an opportunistic pathogen mostly affecting hospitalized and immunodeficient patients. considering the extent of attention paid to the issue of antibiotic resistance and the origin of such resistance and the growing number of treatment failures in patients with burn injury, it seems that investigating the degree of ampc gene expression could be beneficial for...
متن کاملSynergistic Effect of Probiotic Bacteria and Antibiotics on Antibiotic-resistant Strains of Pseudomonas Aeruginosa Isolated from Patients with Burn Wounds
Background: Burn infections are one of the leading causes of death in the world. Antibiotic resistance is a major concern among the medical community. In this study, we investigated the effect of common probiotic strains on multidrug-resistant Pseudomonas aeruginosa strains. Methods: Strains of Pseudomonas aeruginosa from burn wounds of patients were isolated. Then pathogens were biochemically ...
متن کاملOuter Membrane Protein D Gene in Clinical Isolates of Pseudomonas Aeruginosa and its Role in Antibiotic Resistance
Background & Objectives: Pseudomonas aeruginosa is a common cause of nosocomial infection. OprD protein is a specific protein regulating the uptake of carbapenem antibiotic. Loss of OprD is the main mechanism of Pseudomonas Aeruginosa resistance to carbapenem. In this study, the presence of OprD gene is investigated in isolated Pseudomonas Aeruginosa in burn patients of Ghotboddin hospital in S...
متن کاملPLASMID MEDIATED METAL AND ANTIBIOTIC RESISTANCE IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM BURN PATIENTS
Pseudomonas aeruginosa is a leading cause of bum infections, and antibiotic- resistant strains of this bacterium are emerging due to extensive application of antibiotics in the bum unit of hospitals. In this study 50 strains of P. aeruginosa were isolated from bum patients infected with this micro-organism in the bum unit of a general hospital in Kerman, Iran over one year [May 1999-ApriI20...
متن کاملDetection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran
Introduction: Pseudomonas aeruginosa is an opportunistic pathogen of clinical importance, particularly in immunocompromised and burn patients. This bacterium is becoming resistant to many antibiotics via intrinsic or acquired mechanisms. Mutations in anti-mutator genes, such as pfpI, can be a potential intrinsic mechanism of antibiotic resistance. This study aimed to evaluate the possible effec...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of biotechnology and health sciencesجلد ۲، شماره ۳، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023